Особенности разложения моногидроксибифенилов аэробными штаммами, изолированными из бактериальных ассоциаций–деструкторов ароматических поллютантов

Штаммы Micrococcus sp. PNS1, Ochrobactrum sp. PNS5, Stenotrophomonas sp. PNS6, Pseudomonas sp. PNB3, Brevibacterium sp. PNB5, Achromobacter sp. PNB6 и Bosea sp. PNB7, выделенные из ассоциаций PN2 и PN2-B, осуществляют разложение моно-гидроксилированных бифенилов, содержащих в качестве заместителя гидрокси-группу у 3 или 4 атома в молекуле бифенила. Максимальная эффективность деструкции 3-гидроксибифенила составляла 98 %, а 4-гидроксибифенила – 100 % у разных штаммов. У всех исследуемых штаммов был амплифицирован фрагмент гена bphA, кодирующего биосинтез бифенил-2,3-диоксигеназы. Показано, что последовательности гена bphA, полученные в настоящем исследовании, формируют самостоятельную ветвь на графической модели, отображающей их уровень сходства с известными последовательностями данного гена. У штамма Pseudomonas sp. PNB3 выявлено наличие гена benA, кодирующего биосинтез бензоат-1,2-диоксигеназы, и располагающегося в одной ветви эволюционного дерева с геном benA известного штамма-деструктора Pseudomonas putida KT2440. Анализ полученных результатов показал, что трансформация 3-гидрокси- и 4-гидроксибифенилов исследуемыми штаммами происходит в результате диоксигенирования незамещенного кольца молекулы бифенила с последующим расщеплением на 3- и 4-гидроксибензойные кислоты соответственно. Таким образом, исследованные штаммы являются перспективными для применения в технологиях разложения не только хлорбифенилов, но и гидроксилированных бифенилов.

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