Роль клеток-предшественников печени амфибий вида Rana terrestris в постэмбриональном развитии в условиях физиологической нормы

Центром метаболических реакций в организме животных является печень, которая также функционирует как «периферийный интегратор» энергетической потребности организма. Этот орган характеризуется высоким регенераторным потенциалом, который поддерживается за счет пролиферации гепатоцитов, а также гемопоэтических и региональных прогениторных клеток-предшественников печени (LPC). В связи с этим настоящее исследование посвящено изучению роли клеток-предшественников в регенерации печени амфибий вида Rana terrestris в условиях физиологической нормы в постэмбриогенезе. Анализ внутрипеченочных и гемопоэтических маркеров методами иммуногистохимии и проточной цитофлуориметрии показал, что на первый год постэмбриогенеза процессы регенерации печени в равной степени осуществляются за счет клеток-предшественников с иммунофенотипом СК19⁺ (внутрипеченочные клетки-предшественники), а также CD34⁺CD45⁺ (популяция гемопоэтических клеток-предшественников) и CD34⁺CD45^– (популяция гемангиобластов). На второй и третий год постэмбриогенеза основным источником регенерации печени являются СК19⁺-позитивные клетки и в меньшей мере гемопоэтические источники с иммунофенотипом CD34⁺CD45^–. Полученные данные во многом определяются средой обитания амфибий, терморегуляцией, а также завершением процессов морфогенеза на третий год постэмбриогенеза. Печень остается на изучаемые сроки постэмбриогенеза органом гемопоэза.

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