Анализ и апробация методов отделения микроводорослей от культуральной среды, применимых для Porphyridium purpureum
https://doi.org/10.26907/2542-064X.2025.2.312-335
Аннотация
Описаны современные методы отделения биомассы микроводорослей от культуральных сред, а также основные преимущества и недостатки, связанные с их использованием. При выборе наиболее подходящего и экономически обоснованного метода сбора урожая микроводорослей особое внимание следует уделять масштабам производства, видам микроводорослей, составу питательных сред. Проведена апробация методов центрифугирования, гравитационного осаждения и сепарирования для отделения клеток морской микроводоросли Porphyridium purpureum от культуральной среды. Экспериментально установлено значимое превосходство метода сепарирования над двумя другими способами по сухому весу биомассы и по затраченному времени. При энергоемкости в 1 кВт метод сепарирования дает возможность обработать в 100 раз больший объем суспензии и получить 20.75 г сухой биомассы P. purpureum, что демонстрирует пятикратное превосходство над методом центрифугирования. Для обеспечения высокого коэффициента концентрирования биомассы и снижения энергетических затрат рекомендуется применять многостадийный процесс сбора P. purpureum, сочетающий первичное гравитационное осаждение и методы центрифугирования или сепарирования. Проведенные исследования могут служить основой для разработки практических рекомендаций по эффективному сбору микроводорослей в промышленных масштабах.
При поддержке: Работа выполнена при финансовой поддержке Российского научного фонда (проект № 24-26-20131)
Об авторах
С. Ю. Горбунова
Институт биологии южных морей им. А.О. Ковалевского РАН
Россия
Конфликт интересов:
Россия
Светлана Юрьевна Горбунова - кандидат биологических наук, старший научный сотрудник отдела биотехнологий и фиторесурсов.
Севастополь
Конфликт интересов:
Авторы заявляют об отсутствии конфликта интересов
А. Б. Боровков
Институт биологии южных морей им. А.О. Ковалевского РАН
Россия
Конфликт интересов:
Россия
Боровков Андрей Борисович - кандидат биологических наук, заведующий отделом биотехнологий и фиторесурсов.
Севастополь
Конфликт интересов:
Авторы заявляют об отсутствии конфликта интересов
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Рецензия
Для цитирования:
Горбунова С.Ю., Боровков А.Б. Анализ и апробация методов отделения микроводорослей от культуральной среды, применимых для Porphyridium purpureum. Ученые записки Казанского университета. Серия Естественные науки. 2025;167(2):312-335. https://doi.org/10.26907/2542-064X.2025.2.312-335
For citation:
Gorbunova S.Yu., Borovkov A.B. Analysis and evaluation of methods used for harvesting microalgae from culture media and suitable for Porphyridium purpureum. Uchenye Zapiski Kazanskogo Universiteta Seriya Estestvennye Nauki. 2025;167(2):312-335. (In Russ.) https://doi.org/10.26907/2542-064X.2025.2.312-335
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