Avian cell-free translation system based on the cell extract of Gallus gallus embryos
https://doi.org/10.26907/2542-064X.2025.2.297-311
Abstract
Cell-free translation systems are gaining increasingly widespread use, from both practical and fundamental standpoints. Their applications are diverse but typically revolve around preparative biosynthesis of proteins in cases where expression in living cells is either problematic or unfeasible. They also enable rapid evaluation of the effects produced by external components on the translation process. Existing cell-free systems have been derived from bacterial, yeast, plant, insect, mammalian, and human cells. However, no cell-free systems have been developed from avian cells, despite the ecological and economic significance of birds (in daily life, food production, light industry, agriculture, etc.). Such systems would be powerful biotechnological tools and bring considerable benefits for both poultry farming and fundamental research on the protein synthesis in birds. To address this gap, a cell-free translation system using the extracts from the cells of domestic chicken (Gallus gallus) embryos was developed. Following the sample preparation and mRNA selection, the cell-free biosynthesis of firefly luciferase was performed in a chemically supplemented cell extract.
Supporting agencies: This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. 075-15-2021-1344)
About the Authors
A. G. Bikmullin
Kazan Federal University
Russian Federation
Competing Interests:
Russian Federation
Aydar G. Bikmullin - Cand. Sci. (Biology), Senior Researcher, Laboratory of Structural Biology.
Kazan
Competing Interests:
The authors declare no conflicts of interest
E. A. Klochkova
FRC Kazan Scientific Center of the Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
Evelina A. Klochkova - Senior Researcher, Laboratory for Structural Analysis of Biomacromolecules.
Kazan
Competing Interests:
The authors declare no conflicts of interest
N. M. Alexandrova
FRC Kazan Scientific Center of the Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
Natalya M. Alexandrova - Cand. Sci. (Biology), Senior Researcher, Laboratory of Molecular Genetics and Microbiological Methods.
Kazan
Competing Interests:
The authors declare no conflicts of interest
K. S. Usachev
Kazan Federal University; FRC Kazan Scientific Center of the Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
Konstantin S. Usachev - Dr. Sci. (Physics and Mathematics), Head of the Laboratory for Structural Analysis of Biomacromolecules.
Kazan
Competing Interests:
The authors declare no conflicts of interest
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For citations:
Bikmullin A.G., Klochkova E.A., Alexandrova N.M., Usachev K.S. Avian cell-free translation system based on the cell extract of Gallus gallus embryos. Uchenye Zapiski Kazanskogo Universiteta Seriya Estestvennye Nauki. 2025;167(2):297-311. (In Russ.) https://doi.org/10.26907/2542-064X.2025.2.297-311
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